Biancalana was a member of the Royals team that won the World Series in 1985. Although he had only started 35 games all season, manager Dick Howser benched regular shortstop Onix Concepcion in favor of Biancalana on September 20. Biancalana started 13 of the next 15 games as the Royals won the American League West division by one game over the California Angels.
Biancalana was the starting shortstop for the Royals in all 14 post-season games, playing error-free defense in every game, and was an integral part in several run-scoring innings for the Royals in the World Series. He batted .278 in the series with an on-base percentage of .435, both well above his career numbers